Diagnostic Tests

2019-20 Schedule of Autoantibody Tests and Turnaround Times

Please note: all tests ordered can be done on serum and/or CSF unless otherwise indicated below

Name of Test/AssayProcedure/TechniqueTurnaround time (Days after sample received)
ADAMTS-13 Activity & INH Profile:

• ADAMTS-13 Activity

• ADAMTS-13 Auto-Antibodies/Inhibitors


ELISA

ELISA


1-2

1-2
Anti-Cellular AntibodiesIIF on HEp-2 substrate with pattern interpretation5
Anti-Histone:
Drug Induced Lupus

LIA

5-7
Anti-phospholipid Syndrome:

• Anti-Domain 1 B2GP1

• Phosphatidylserine/ Prothrombin Complex (PS/PT): IgG,
IgM


CIA

ELISA


5

5
Arthritis PanelALBIA5
Cancer Associated Autoantibody PanelALBIA (LDT)14
Cytoplasmic Dot ProfileALBIA (LDT)5-7
Dense Fine Speckled ANA Pattern (Anti-DFS70 Target)CIA5-7
Dermatology:
Bullous Autoimmune Skin Disease Profile

CBA

5-7
DNA Antibodies:

• Anti-single stranded DNA

• Anti-dsDNA


ELISA

CIA


5

5
Gastrointestinal:
Inflammatory Bowel Disease ASCA: IgG, IgA

ELISA

5
Liver:

• Autoimmune Liver Disease Profile ** Serum only

• Autoimmune Liver Disease 14 Profile PLUS ** Serum only


LIA

LIA


5-7

5-7
Lung:

• Interstitial Lung Disease Profile (ILD)

• Alveolar Proteinases: anti-GMCSF


LIA, CIA

ALBIA (LDT)


5-7

5-7
Muscle:

• Autoimmune Myopathy / Myositis Profile PLUS (Includes Mup44 & Immune Mediated Necrotizing Profile)

• Anti-SMN / Gemin1


LIA, ELISA, ALBIA (LDT)

ALBIA (LDT)


5-7

5-7
Myasthenia Gravis:
Anti-Acetylcholine Receptor (AChR)

ELISA

5-7
Nephritis:

• Idiopathic Membranous Nephropathy:
Anti-PLA2R

• Primary Membranous Nephropathy:
Anti-THSD7A (Thrombospondin)


Uromodulin Level


CBA


CBA



ELISA


5-7


5-7



5-7
Neurofilament (pNf-H)ELISA7
Neurological Disease Profile (IgG, IgM) **Serum onlyLIA5-7
Neurological:
• Anti-IgLON5
CBA5-7
Neurological: Anti-GAD65CBA5-7
Neurological: Anti-Myelin Associated Glycoproteins LIA5-7
Neurological: Encephalitis (AI ENC)

• NMDA (NR1) Receptor Antibodies

• Anti-DPPX

• Anti-GABAB Receptor

• VGKC Antibodies (LGI1 & CASPR2)

• Anti-AMPA Receptor


Neuromyelitis Spectrum Profile


CBA

CBA

CBA

CBA

CBA


CBA


5-7

5-7

5-7

5-7

5-7


5-7
Neurological:
Idiopathic Ataxia
ALBIA (LDT)5-7
Neurological/Cancer:

• Paraneoplastic Disease Profile

• Paraneoplastic Neurological Syndrome PLUS


LIA

LIA


5-7

5-7
Nuclear Envelope/Membrane ProfileLIA5-7
Nucleolar Autoantibody ProfileIFA, LIA5-7
Scleroderma/Systemic Sclerosis ProfileLIA5-7
Sjögren’s Syndrome ProfileLIA5-7
Systemic Lupus profileLIA/ELISA5-7
Vasculitis:

ANCA/PR3, MPO quantitative by CIA

Anti-P140/P155/TRIM28

Atypical ANCA by Multiplex: Anti-LAMP2, Anti-Elastase


CIA

ALBIA (LDT)

ALBIA (LDT)


5-7

5-7

5-7

 

** LDT (Laboratory Developed Test)

** Price increases primarily reflect increase in the price charged by kit manufacturers. Other increases reflect smaller changes due to increased costs of labour and supplies.

 

Abbreviations:

AIH: autoimmune hepatitis; ALBIA: addressable laser bead immunoassay by Luminex; AMPAR: (a-amino-3-hydroxy-5-mehtyl-4-isoxasole proprionate receptor): limbic encephalitis; ANA: antinuclear antibody; ANCA, anti-cytoplasmic neutrophil antibody; ASCA: anti-Saccharomyces cerevisiae antibody; CBA: cell based assay; CENP: centromere protein; CIA: chemiluminescence assay (BioFlash); DFS, dense fine speckled; DPPX: dipeptidyl peptidase like 6; EEA-1: early endosome antigen 1; ELISA: enzyme linked immunosorbent assay; GAD: glutamate decarboxylase; GM-CSF: granulocyte macrophage colony stimulating factor; IBD: inflammatory bowel disease; IIF: indirect immunofluorescence; LAMP, lysosome associated membrane protein; LDT, laboratory developed test; LIA: line immunoassay; MAG: myelin associate glycoprotein; MecP2: methyl CpG binding protein 2; MPO: myeloperoxidase; MPP: M-phase phosphoprotein 1; NMDA: N-methyl-D-aspartic acid; NMO: neuromyelitis optica spectrum (Devic’s Diseases); PBC: primary biliary cirrhosis; pNf-H; phosphorylated heavy chain  neurofilament H; PR3: proteinase 3; PS/PT: phosphatidyl serine/prothrombin complex; SARD: systemic autoimmune rheumatic diseases (SLE, scleroderma, myositis, Sjögren’s syndrome); SLE: systemic lupus erythematosus; SMN, survival of motor neuron; THSD7A, thrombospondin type-1 domain-containing protein 7A; TRIM, tri-partite motif; VGKC: voltage gated potassium channel.

 

Disease and Autoantibody Related Panels/Profiles:

  • Anti‐Cellular Antibodies: Atypical and Cell Cycle Patterns
  • Arthritis Panel: Anti‐Citrullinated peptides – anti‐ HCP1, HCP2, VCP1 and VCP2. Rheumatoid Factor   (IgM) IgA available
  • Autoimmune Liver Disease Profile: AMA-M2, M2-3EBPO, LKM, SLA, SP100, gp210, PML, LC‐1, Ro 52/TRIM21
  • Autoimmune Liver 14 Profile PLUS: AMA-M2, M2-3E(BPO), LKM, SLA, SP100, gp210, PML, LC‐1, Ro 52/TRIM21, Scl-70, Centromere A, Centromere B, PGDH
  • Autoimmune Myopathy / Myositis Profile PLUS (Includes Mup44 & Immune Mediated Necrotizing Profile): Jo‐1, Mi2, Mi2‐α, Mi2β, MDA5, NXP2, TIF1γ PL7, PL12, PM/Scl75, PM/Scl100, Ku, SRP, EJ, OJ, Ro52, HMGCR, anti-NT5C1 A/Mup44
  • Bullous Autoimmune Skin Disease Profile: BP180, BP230, Desmoglein 1, Desmoglein 3
  • Cancer Associated Autoantibody Panel: CENP-F, p53 (LDT)
  • Cytoplasmic Dot Profile: GW Bodies (Ge‐1, GW182, Ago‐2); EEA1 (LDT)
  • Interstitial Lung Disease Profile: Anti‐CENP A + B, Topo‐I/Scl‐70, RNA polymerase III, fibrillarin, Th/To/hPOP1, Ku, PDGFR, Ro52/TRIM21, PM/Scl‐75, PM/Scl-100, Nor90/Hubf, Jo‐1, Mi2, Mi2‐α, Mi2β, MDA5, NXP2, TIF1γ PL7, PL12, Ku, SRP, EJ, OJ
  • Neurological Disease Profile (IgG +IgM): Anti‐GM1, GM2, GM3, GD1a, GD1b, GT1b, GQ1b
  • Neuromyelitis Spectrum Profile: Anti-Aquaporin 4/ Anti-Myelin Oligodendrocyte Glycoproteins
  • Paraneoplastic Disease Profile: Amphiphysin, Ri (NOVA‐1), Yo, Hu, PNMA2 (Ma2/Ta), CV2.1, Recoverin, SOX1, Titin
  • Paraneoplastic Neurological Syndrome PLUS: Amphiphysin, Ri (NOVA‐1), Yo, Hu, PNMA2 (Ma2/Ta), CV2.1, Recoverin, SOX1, Titin, Zic4, GAD65, Tr (DNER)
  • Scleroderma/Systemic Sclerosis Profile: Anti‐CENP A + B, Topo‐I/Scl‐70, RNA polymerase III, fibrillarin/U3RNP, Th/To/hPOP1, Ku, PDGFR, Ro52/TRIM21, PM/Scl‐75, PM/Scl‐100, NOR90/Hubf
  • Sjögren’s Syndrome Profile: Anti‐SS‐A/Ro, SS‐B/La, anti‐Ro52/TRIM21
  • Systemic Lupus Profile: Anti-Sm, U1RNP, Ro52/TRIM21, SSA/Ro60, SSB/La, PCNA, dsDNA, Ku, Ribosomal-P and Chromatin

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